- Definition: A not very great way to deliver gene editors to cells
- Schematic
- Downsides
- Causes cell toxicity
- Requires hardware (hard to scale up)
- Cell loss associated w/ washing/tubing (moving the cell thru tubes)
- Does not allow for safe multiplexing (targeting multiple genes or genetic sites and cutting simultaneously in a single cell) / sequential editing ⇒ bc causes translocations